We support phage research and development of phage-based therapeutics:
- Quantitative determination of phage proteins and the stoichiometry of virions
- Quality control of therapeutics (HCP detection and quantification)
- GMP-conform procedures
Ebhardt et al. Proteomics. 2015 Sep;15(18):3193-208
Antimicrobial resistance has increased over the past decades and the reducing efficiency of antibiotics demands for new treatments. Phages are natural antibacterial agents and phage research has received a lot of attention, especially its therapeutic application.
As for other biologically derived therapeutics phage-based products demand for sophisticated quality control.
Mass spectrometry enables the identification of thousands of proteins in one measurement, enabling the unbiased detection of host contaminant proteins in therapeutic phages and viral vaccines at low setup costs. Our proprietary QconCAT technology allows for quantitative determination of phage proteins and the stoichiometry of virions, measuring up to 50 virus proteins with a single QconCAT reference standard.
For example, covering the proteome of phiKZ would demand for 4-6 QconCATs, saving you time preparing complex peptide mixes. Additionally, there is no need for production of specific antibodies, enabling fast assay development.
Quantification of phage proteins
Time-resolved protein expression of viral proteins and/or selected host proteins
Stoichiometry of whole phages
Highly sensitive detection of HCPs
Highly accurate quantification of selected HCPs
Low per sample cost
Measurement in complex matrices (multiple phages, disturbing chemicals, phage proteins in host cells)
Fast and robust measurements for routine analyses
Comprehensive analysis, performed and evaluated by experiences professionals