RePLiCal

A retention time standard for HPLC instrument calibration

Order Number Product Package size Price (€)
PQ-CS-1560 RePLiCal – complete protein, lyophilized 1 µg € 189,-
PQ-CS-1561 RePLiCal – peptide mix, lyophilized, digested 1 µg € 199,-
PQ-CS-1561S RePLiCal – peptide mix, lyophilized, digested 200 ng € 59,-

Order Information

You can place your RePLiCal order here or return the completed order form. You can also contact us directly through:

E-Mail

Phone: +49 (0)9405 96999 10

or Fax: +49 (0)9405 96999 28

Our prices are net prices, i.e. they do not contain value added taxes or customs fees. These taxes have to be paid by the customer.
The ordered products are usually shipped 1-2 business days after receiving the order.
The costs of the delivery have to be paid by the customer. Please select your preferred shipping option

Destination Option Delivery time Shipping costs (€)
Germany DHL standard 2-3 days 5,-
Germany DHL express 1 day 10,-
EU DHL standard 4-12 days 10,-
International FEDEX international priority 3-7 days 25-55,-
depends on destination

If you have questions about shipping please contact us.

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Product Information

RePLiCal calibration standards for HPLC
  • Higher number of calibrant points than other commercially available peptide standard kits
  • Stable RTs over long analysis times allow the prediction of RTs for other peptides in a timely manner
  • Includes two highly hydrophilic peptides to allow testing of column trapping performance

RePLiCal is a HPLC calibration standard that is buildt from 27 lysine-terminating calibrant peptides. The peptides do not contain methionine, tryptophan, cysteine, and N-terminal glutamine residues. Also aspartic acid-proline, asparagine-proline and asparagine-glycine motifs are absent. It can be applied to calibration of LC gradients and nano uHPLC instruments using trap-elute configurations, real-time scheduling of selected reaction monitoring (SRM) experiments and recalibration of the m/z scale of mass spectrometry (MS) data post-acquisition. Find more information in the original publication.

Application

1 µg RePLiCal or 25510 fmol (digested or undigested) equals 250 – 500 injections (50 – 100 fmol/injection).

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