RePLiCal
Retention time standard for HPLC instrument calibration
RePLiCal comprises of unique, easy to detect peptides with selected chemical properties.
- High number of calibrant points, covering a broad range of RTs
- Stable RTs over long analysis times, allowing the prediction of RTs for other peptides in a timely manner
- Includes two highly hydrophilic peptides for testing of column trapping performance
| Order Number | Product | Package size | Application | Price (€) | |
| PQ-CS-1560 | RePLiCal – complete protein, lyophilized | 1 µg | 250-500 injections | 189.00 |  |
| PQ-CS-1561 | RePLiCal – peptide mix, lyophilized, digested | 1 µg | 250-500 injections | 199.00 | |
| PQ-CS-2560 | RePLiCal – complete protein, 13C,15N Lys/Arg-labelled, lyophilized | 1 µg | 250-500 injections | 209.00 | |
| PQ-CS-2561 | RePLiCal – peptide mix, 13C,15N Lys/Arg-labelled, lyophilized, digested | 1 µg | 250-500 injections | 219.00 | |
RePLiCals peptides have a high degree of linearity and thus can be used to predict the retention times of the peptides on a new gradient. By calculating iRTs, retention time windows can be adjusted for different gradients.
This tutorial demonstrates how RePLiCal can be used to re-calculate retention times using Skyline.
Order Information
You can place your RePLiCal order here or return the completed order form. You can also contact us directly through:
Phone: +49 (0)9405 96999 10
or Fax: +49 (0)9405 96999 28
Our prices are net prices, i.e. they do not contain value added taxes or customs fees. These taxes have to be paid by the customer.
Ordered products are usually shipped 1-2 business days after receiving the order.
The costs of the delivery have to be paid by the customer. Please select your preferred shipping option
| Destination | Option | Delivery time | Shipping costs (€) |
| Germany | DHL standard | 2-3 days | 5,- |
| Germany | DHL express | 1 day | 10,- |
| EU | DHL standard | 4-12 days | 10,- |
| International | FEDEX international priority | 3-7 days | 25-55,- depends on destination |
If you have questions about shipping please contact us.
Product Information
RePLiCal is a HPLC calibration standard that is buildt from 27 lysine-terminating calibrant peptides. The peptides do not contain methionine, tryptophan, cysteine, and N-terminal glutamine residues. Also aspartic acid-proline, asparagine-proline and asparagine-glycine motifs are absent. It can be applied to calibration of LC gradients and nano uHPLC instruments using trap-elute configurations, real-time scheduling of selected reaction monitoring (SRM) experiments and recalibration of the m/z scale of mass spectrometry (MS) data post-acquisition. Find more information in the original publication.
Separation was performed on Zorbax 300SB-C18 (5×0.075mm, 3.5µm p. s.) with gradient elution at 350 nl/min from 8%-38% B (solvent A: 5% acetonitrile/0.1% formic acid in water, solvent B: 0.1% formic acid in acetonitrile. Total run time was 60 min.
Survey spectra were recorded in a range from m/z 300 to m/z 1600 using an Orbitrap XL.
Triplicate measurements; error bars: ±2 standard deviations. High confidence in linearity is also given for very long gradients (even for 480 min).
Application
1 µg RePLiCal or 25510 fmol (digested or undigested) equals 250 – 500 injections (50 – 100 fmol/injection).
Watch our tutorial to learn „How to use the RePLiCal RT standard to adapt for RT shifts in Skyline“.
Downloads
References
- RePLiCal: A QconCAT Protein for Retention Time Standardization in Proteomics Studies. Holman S. et al., J Proteome Res. 2016 Mar 4;15(3):1090-102. [PubMed]
- Platform Independent Protein-Based Cell-Of-Origin Subtyping of Diffuse Large B-cell Lymphoma in Formalin-Fixed Paraffin-Embedded Tissue. Reinders J., et al., Sci Rep. 2020 May 12;10(1):7876. [PubMed]