A retention time standard for HPLC instrument calibration
|Order Number||Product||Package size||Price (€)|
|PQ-CS-1560||RePLiCal – complete protein, lyophilized||1 µg||€ 189,-|
|PQ-CS-1561||RePLiCal – peptide mix, lyophilized, digested||1 µg||€ 199,-|
|PQ-CS-1561S||RePLiCal – peptide mix, lyophilized, digested||200 ng||€ 59,-|
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|Destination||Option||Delivery time||Shipping costs (€)|
|Germany||DHL standard||2-3 days||5,-|
|Germany||DHL express||1 day||10,-|
|EU||DHL standard||4-12 days||10,-|
|International||FEDEX international priority||3-7 days||25-55,-
depends on destination
If you have questions about shipping please contact us.
- Higher number of calibrant points than other commercially available peptide standard kits
- Stable RTs over long analysis times allow the prediction of RTs for other peptides in a timely manner
- Includes two highly hydrophilic peptides to allow testing of column trapping performance
RePLiCal is a HPLC calibration standard that is buildt from 27 lysine-terminating calibrant peptides. The peptides do not contain methionine, tryptophan, cysteine, and N-terminal glutamine residues. Also aspartic acid-proline, asparagine-proline and asparagine-glycine motifs are absent. It can be applied to calibration of LC gradients and nano uHPLC instruments using trap-elute configurations, real-time scheduling of selected reaction monitoring (SRM) experiments and recalibration of the m/z scale of mass spectrometry (MS) data post-acquisition. Find more information in the original publication.
1 µg RePLiCal or 25510 fmol (digested or undigested) equals 250 – 500 injections (50 – 100 fmol/injection).
- RePLiCal: A QconCAT Protein for Retention Time Standardization in Proteomics Studies. Holman S. et al., J Proteome Res. 2016 Mar 4;15(3):1090-102. [PubMed]
- Platform Independent Protein-Based Cell-Of-Origin Subtyping of Diffuse Large B-cell Lymphoma in Formalin-Fixed Paraffin-Embedded Tissue. Reinders J., et al., Sci Rep. 2020 May 12;10(1):7876. [PubMed]