This tutorial demonstrates how to use RePLiCal for calculating and re-calculating iRTs in Skyline.

More information can be found here:

  • Skyline webinar #7: iRT Retention Time Prediction with Skyline
  • Using iRT, a normalized retention time for more targeted measurement of peptides. Escher C. et al., Proteomics. 2012 Apr;12(8):1111-21. [PubMed]
  • RePLiCal: A QconCAT Protein for Retention Time Standardization in Proteomics Studies. Holman S. et al., J Proteome Res. 2016 Mar 4;15(3):1090-102. [PubMed]

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