Overview about the most frequently used research technologies in quantitative proteomics.
Method | Advantages | Drawbacks |
QconCAT | Digest control Accurate Multiplexing Cost-effective High stability |
Complex design |
Synthetic peptides (e.g. AQUA) |
Cost-effective | Possible resolubilization problems High work effort preparing peptide mixtures Low stability No digest control |
QPrEST | Cost-effective | Limited peptide selection No custom design High work effort preparing peptide mixtures |
Complete labeled protein | Accurate High stability Digest control | Expensive High work effort for protein expression |