Proteomics methods

Overview about the most frequently used research technologies in quantitative proteomics.

MethodAdvantagesDrawbacks
QconCAT Digest control
Accurate
Multiplexing
Cost-effective
High stability
Complex design
Synthetic peptides
(e.g. AQUA)
Cost-effective Possible resolubilization problems
High work effort preparing peptide mixtures
Low stability
No digest control
QPrESTCost-effective Limited peptide selection
No custom design
High work effort preparing peptide mixtures
Complete labeled protein Accurate
High stability
Digest control
Expensive
High work effort for protein expression

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